Accelerate Vigyan and KIMS Autoantibody workshop
July 25th to 29th 2022
Organised by:
Departments of Clinical Immunology and Rheumatology, KIMS, KIIT University, Institute of Eminence, Bhubaneswar
Course Objectives:
After attending the course, participants should be able to
- Understand the principles and methodology of laboratory techniques (Agglutination, IIF, ELISA, Line blots) employed in carrying out tests for various
- Analysis and Interpretation of laboratory
- Clinical correlation of various autoantibody tests
Course Description:
The course is intended for MD students and Senior Residents in Medicine, Pathology , Microbiology, Dermatology and DM students in Clinical Immunology and Rheumatology
To Cover
Principles and methodology of laboratory techniques; Agglutination, ELISA, indirect immunofluorescence, Line blot assays and PCR and RT PCR
Hands-on training during the workshop will give the participants a feel of the laboratory test and an insight to various laboratory techniques and idea about the common problems that arise in lab and how to troubleshoot them.
All the participants will be provided an opportunity to perform Agglutination, Immunofluorescence assay for ANA, ANCA, ANA and Myositis Specific autoantibodies by Line blot and ELISA for anti ds DNA, cardiolipin and PR3 and MPO assays with their own hands and discuss the results of their tests with the faculty.
These will be followed by lectures on interpretation of tests in patients having various diseases.
Last Date of Application: 23 July 2022
Maximum candidates: 20 (First come First Serve Basis)
Contact:
Dr Sakir Ahmed , Associate Professor Clinical Immunology and Rheumatology
Email: sakir.ahmed @kims.ac.in
Mobile: 9706023644
| Day | Lecture (Lab Techniques) |
TEA | Practical | LUNCH | Practical | TEA | Lecture (Clinical Implications) |
|---|---|---|---|---|---|---|---|
| 9.00 AM -10.00 AM | 10.30 – 12.30 PM | 3.00 – 4.00 PM | 4.00 – 5.00pm | ||||
| 25 Jul Mon |
Antigen antibody interaction (precipitation and agglutination) Prof A Aggarwal |
Nephelometer: sample processing and standard curves | RPR and Widal test; Rheumatoid factor by Latex | Autoantibodies in Rheumatoid arthritis Prof A Aggarwal |
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| 26 Jul Tue |
Anti-nuclear anti bodies: methods of detection Dr SakirAhmed |
Anti nuclear antibody: staining | Anti nuclear antibody: reporting | Autoantibodies in SLE and other connective tissue Diseases Prof P Padhan |
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| 27 Jul Wed |
Immunofluorescence assayson neutrophils, Crithidialuciliae and tissue Prof Pradeepto Patro |
Staining for ANCA and tissue slides | Reporting ANCA IIF and tissue IIF | Autoantibodies in vasculitis Prof D Danda |
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| 28 Jul Thur |
Principles of ELISA Dr R Tripathy |
ELISA for CCP, PR3, MPO, dsDNA, cardiolipin | ELISA for CCP, PR3, MPO, dsDNA | Autoantibodies inprothrombotic state Prof Bidyut Das |
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| 29 Jul Fri |
HLA and genetic testing for SNP Dr Christina M Mariaselvam |
HLA B27 by PCR RT PCR |
Interpreting SNP data | HLA Association in immune rheumaticdiseases Dr Debashish Maikup |
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| 30 Jul Sat |
Immunoblot Dr R Misra |
Line Blot forANA profile and MSA profile | Valedictory function 12 -12.30 |
